![]() After 4 weeks, the adult generation was removed before their offspring finished metamorphosis and DNA of these females extracted and the symbiont titer was analyzed by quantitative PCR (see below 1). ![]() Therefore, 10 female adult beetles were individually separated in a single jar with oat flakes to lay eggs, as were symbiotic beetles in parallel populations. Before the following experiments the aposymbiotic status of this beetle sub-population was confirmed. surinamensis sub-population was treated for 12 weeks with tetracycline (150 mg/5g oat flakes) to eliminate their symbionts and then kept for several generations on a normal diet to exclude direct effects of tetracyclin on the host physiology 1. surinamensis population (strain OsNFRI) was obtained from the National Food Research Institute (Tsukuba, Japan) and used for genome sequencing.Īn O. surinamensis cultures were maintained in 1.8-L plastic containers, filled with 50 g oat flakes, at 28 ☌, 60% relative humidity and a day and night cycles of 16–8 h. Continuous symbiotic and aposymbiotic (see below) O. The initial Oryzaephilus surinamensis culture (strain JKI) was obtained from the Julius-Kühn-Institute/Federal Research Center for Cultivated Plants (Berlin, Germany) in 2014 and kept in culture since then.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |